{"id":454,"date":"2025-01-24T08:58:21","date_gmt":"2025-01-24T07:58:21","guid":{"rendered":"https:\/\/web.umons.ac.be\/card\/?page_id=454"},"modified":"2025-01-24T12:15:02","modified_gmt":"2025-01-24T11:15:02","slug":"tests-de-la-cardiotoxicite-des-traitements-anticancereux-sur-des-spheroides-cardiaques-in-vitro","status":"publish","type":"page","link":"https:\/\/web.umons.ac.be\/card\/tests-de-la-cardiotoxicite-des-traitements-anticancereux-sur-des-spheroides-cardiaques-in-vitro\/","title":{"rendered":"Tests de la cardiotoxicit\u00e9 des traitements anticanc\u00e9reux sur des sph\u00e9ro\u00efdes cardiaques in vitro."},"content":{"rendered":"<p style=\"text-align: justify;\"><span style=\"font-family: arial, helvetica, sans-serif; font-size: 12pt;\">Notre dernier projet s&rsquo;attelle \u00e0 tester la cardiotoxicit\u00e9 de traitements anticanc\u00e9reux sur des sph\u00e9ro\u00efdes cardiaques<em>\u00a0in vitro\u00a0<\/em>d\u00e9velopp\u00e9s au laboratoire (Th\u00e8se d&rsquo;Ana\u00eblle Fratard).<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: arial, helvetica, sans-serif; font-size: 12pt;\">En effet, l&rsquo;\u00e9volution des traitements anticanc\u00e9reux a permis d&rsquo;am\u00e9liorer la qualit\u00e9 de soins des patients mais aussi leur survie. Toutefois, certaines th\u00e9rapies anticanc\u00e9reuses engendrent de la toxicit\u00e9, et plus particuli\u00e8rement de la cardiotoxicit\u00e9. Celle-ci cause sur le d\u00e9veloppement de maladies cardiovasculaires plus ou moins graves, et \u00e0 terme, pour certains patients, la mort.<\/span><\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: arial, helvetica, sans-serif; font-size: 12pt;\">Parmi ces th\u00e9rapies, nous \u00e9tudions certaines chimioth\u00e9rapies et th\u00e9rapies cibl\u00e9es utilis\u00e9es notamment dans le traitement du cancer du sein et montrant de la cardiotoxicit\u00e9 chez ces patients.\u00a0<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-family: arial, helvetica, sans-serif; font-size: 12pt;\">Pour cela, des sph\u00e9ro\u00efdes cardiaques sont d\u00e9velopp\u00e9s \u00e0 partir de cellules cardiaques humaines et seront utilis\u00e9s pour nos tests de toxicit\u00e9.<\/span><\/p>\n<p>&nbsp;<\/p>\n<figure id=\"attachment_463\" aria-describedby=\"caption-attachment-463\" style=\"width: 300px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-463 size-medium\" src=\"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/spheroide-J5-plaque-n\u00b01-300x265.jpg\" alt=\"\" width=\"300\" height=\"265\" srcset=\"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/spheroide-J5-plaque-n\u00b01-300x265.jpg 300w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/spheroide-J5-plaque-n\u00b01-768x679.jpg 768w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/spheroide-J5-plaque-n\u00b01-746x660.jpg 746w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/spheroide-J5-plaque-n\u00b01-30x27.jpg 30w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/spheroide-J5-plaque-n\u00b01-60x53.jpg 60w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/spheroide-J5-plaque-n\u00b01-360x318.jpg 360w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/spheroide-J5-plaque-n\u00b01.jpg 855w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><figcaption id=\"caption-attachment-463\" class=\"wp-caption-text\">Photographie d&rsquo;un sph\u00e9ro\u00efde au microscope optique<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p style=\"text-align: justify;\"><span style=\"font-family: arial, helvetica, sans-serif; font-size: 12pt;\">Par la suite, les sph\u00e9ro\u00efdes cardiaques sont r\u00e9colt\u00e9s puis mis en paraffine. Des coupes sont ensuite r\u00e9alis\u00e9es puis une coloration H\u00e9matoxyline \u00e9osine est r\u00e9alis\u00e9e pour observer la structure des sph\u00e9ro\u00efdes au microscope optique.<\/span><\/p>\n<p>&nbsp;<\/p>\n<figure id=\"attachment_467\" aria-describedby=\"caption-attachment-467\" style=\"width: 333px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-467\" src=\"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-300x156.png\" alt=\"\" width=\"333\" height=\"173\" srcset=\"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-300x156.png 300w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-1024x532.png 1024w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-768x399.png 768w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-1536x798.png 1536w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-2048x1064.png 2048w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-1271x660.png 1271w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-30x16.png 30w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-60x31.png 60w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-360x187.png 360w\" sizes=\"auto, (max-width: 333px) 100vw, 333px\" \/><figcaption id=\"caption-attachment-467\" class=\"wp-caption-text\">Coupe d&rsquo;un sph\u00e9ro\u00efde color\u00e9 \u00e0 l&rsquo;h\u00e9matoxyline \u00e9osine<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-family: arial, helvetica, sans-serif; font-size: 12pt;\">Nous mettons \u00e9galement en \u00e9vidence les sph\u00e9ro\u00efdes <span style=\"font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400;\">en utilisant un marqueur nucl\u00e9aire (en bleu)<\/span> en fluorescence. Ce fut tr\u00e8s cocasse d&rsquo;observer pour la premi\u00e8re fois un sph\u00e9ro\u00efde cardiaque ayant &#8230; la forme d&rsquo;un c\u0153ur !<\/span><\/p>\n<p>&nbsp;<\/p>\n<figure id=\"attachment_470\" aria-describedby=\"caption-attachment-470\" style=\"width: 300px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-470\" src=\"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/IF-plaque-n\u00b01-300x249.png\" alt=\"\" width=\"300\" height=\"249\" srcset=\"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/IF-plaque-n\u00b01-300x249.png 300w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/IF-plaque-n\u00b01-768x637.png 768w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/IF-plaque-n\u00b01-796x660.png 796w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/IF-plaque-n\u00b01-30x25.png 30w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/IF-plaque-n\u00b01-60x50.png 60w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/IF-plaque-n\u00b01-360x298.png 360w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/IF-plaque-n\u00b01.png 878w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><figcaption id=\"caption-attachment-470\" class=\"wp-caption-text\">Sph\u00e9ro\u00efde avec un marqueur nucl\u00e9aire (en bleu) au microscope confocal<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-family: arial, helvetica, sans-serif; font-size: 12pt;\">De nouveaux tests sont en cours. Restez connect\u00e9s !<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Notre dernier projet s&rsquo;attelle \u00e0 tester la cardiotoxicit\u00e9 de traitements anticanc\u00e9reux sur des sph\u00e9ro\u00efdes cardiaques\u00a0in vitro\u00a0d\u00e9velopp\u00e9s au laboratoire (Th\u00e8se d&rsquo;Ana\u00eblle Fratard). En effet, l&rsquo;\u00e9volution des traitements anticanc\u00e9reux a permis d&rsquo;am\u00e9liorer la qualit\u00e9 de soins des patients mais aussi leur survie. Toutefois, certaines th\u00e9rapies anticanc\u00e9reuses engendrent de la toxicit\u00e9, et plus particuli\u00e8rement de la cardiotoxicit\u00e9. Celle-ci [&hellip;]<\/p>\n","protected":false},"author":83,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-454","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.2 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Tests de la cardiotoxicit\u00e9 des traitements anticanc\u00e9reux sur des sph\u00e9ro\u00efdes cardiaques in vitro. - Service \/ FMPB - Cardiologie<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/web.umons.ac.be\/card\/tests-de-la-cardiotoxicite-des-traitements-anticancereux-sur-des-spheroides-cardiaques-in-vitro\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Tests de la cardiotoxicit\u00e9 des traitements anticanc\u00e9reux sur des sph\u00e9ro\u00efdes cardiaques in vitro. - Service \/ FMPB - Cardiologie\" \/>\n<meta property=\"og:description\" content=\"Notre dernier projet s&rsquo;attelle \u00e0 tester la cardiotoxicit\u00e9 de traitements anticanc\u00e9reux sur des sph\u00e9ro\u00efdes cardiaques\u00a0in vitro\u00a0d\u00e9velopp\u00e9s au laboratoire (Th\u00e8se d&rsquo;Ana\u00eblle Fratard). En effet, l&rsquo;\u00e9volution des traitements anticanc\u00e9reux a permis d&rsquo;am\u00e9liorer la qualit\u00e9 de soins des patients mais aussi leur survie. Toutefois, certaines th\u00e9rapies anticanc\u00e9reuses engendrent de la toxicit\u00e9, et plus particuli\u00e8rement de la cardiotoxicit\u00e9. 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