{"id":458,"date":"2025-01-24T08:59:58","date_gmt":"2025-01-24T07:59:58","guid":{"rendered":"https:\/\/web.umons.ac.be\/card\/?page_id=458"},"modified":"2025-01-24T12:18:26","modified_gmt":"2025-01-24T11:18:26","slug":"testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro","status":"publish","type":"page","link":"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/","title":{"rendered":"Testing the cardiotoxicity of anti-cancer treatments on cardiac spheroids in vitro."},"content":{"rendered":"<p>Our latest project is testing the cardiotoxicity of some anti-cancer treatments on <em>in vitro<\/em> cardiac spheroids developed in the laboratory (Ana\u00eblle Fratard&rsquo;s thesis).<\/p>\n<p>Developments in cancer treatments have improved the quality of patient care and their survival. However, some anti-cancer therapies are associated with toxicity, particularly cardiotoxicity. This leads to the development of cardiovascular disease of varying degrees of severity, and ultimately, for some patients, death.<\/p>\n<p>Among these therapies, we are studying certain chemotherapies and targeted therapies used in particular in the treatment of breast cancer and showing cardiotoxicity in these patients.<\/p>\n<p>To this end, cardiac spheroids are being developed from human heart cells and will be used for our toxicity tests.<\/p>\n<p>&nbsp;<\/p>\n<figure id=\"attachment_463\" aria-describedby=\"caption-attachment-463\" style=\"width: 300px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-463 size-medium\" src=\"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/spheroide-J5-plaque-n\u00b01-300x265.jpg\" alt=\"\" width=\"300\" height=\"265\" srcset=\"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/spheroide-J5-plaque-n\u00b01-300x265.jpg 300w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/spheroide-J5-plaque-n\u00b01-768x679.jpg 768w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/spheroide-J5-plaque-n\u00b01-746x660.jpg 746w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/spheroide-J5-plaque-n\u00b01-30x27.jpg 30w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/spheroide-J5-plaque-n\u00b01-60x53.jpg 60w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/spheroide-J5-plaque-n\u00b01-360x318.jpg 360w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/spheroide-J5-plaque-n\u00b01.jpg 855w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><figcaption id=\"caption-attachment-463\" class=\"wp-caption-text\">Optical microscope photograph of a spheroid<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>The cardiac spheroids were then harvested and embedded in paraffin. Sections were then cut and a haematoxylin and eosin stain was applied to observe the structure of the spheroids under the light microscope.<\/p>\n<p>&nbsp;<\/p>\n<figure id=\"attachment_467\" aria-describedby=\"caption-attachment-467\" style=\"width: 300px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-467\" src=\"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-300x156.png\" alt=\"\" width=\"300\" height=\"156\" srcset=\"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-300x156.png 300w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-1024x532.png 1024w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-768x399.png 768w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-1536x798.png 1536w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-2048x1064.png 2048w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-1271x660.png 1271w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-30x16.png 30w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-60x31.png 60w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-360x187.png 360w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><figcaption id=\"caption-attachment-467\" class=\"wp-caption-text\">Section of spheroids stained with haematoxylin and eosin<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>We are also highlighting the spheroids using a nuclear marker (in blue) in fluorescence. It was very funny to observe for the first time a cardiac spheroid in the shape of &#8230; a heart!<\/p>\n<p>&nbsp;<\/p>\n<figure id=\"attachment_470\" aria-describedby=\"caption-attachment-470\" style=\"width: 300px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-470\" src=\"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/IF-plaque-n\u00b01-300x249.png\" alt=\"\" width=\"300\" height=\"249\" srcset=\"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/IF-plaque-n\u00b01-300x249.png 300w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/IF-plaque-n\u00b01-768x637.png 768w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/IF-plaque-n\u00b01-796x660.png 796w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/IF-plaque-n\u00b01-30x25.png 30w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/IF-plaque-n\u00b01-60x50.png 60w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/IF-plaque-n\u00b01-360x298.png 360w, https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/IF-plaque-n\u00b01.png 878w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><figcaption id=\"caption-attachment-470\" class=\"wp-caption-text\">Spheroid with a nuclear marker (in blue) under a confocal microscope<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>Further tests are currently under way. Stay tuned!<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Our latest project is testing the cardiotoxicity of some anti-cancer treatments on in vitro cardiac spheroids developed in the laboratory (Ana\u00eblle Fratard&rsquo;s thesis). Developments in cancer treatments have improved the quality of patient care and their survival. However, some anti-cancer therapies are associated with toxicity, particularly cardiotoxicity. This leads to the development of cardiovascular disease [&hellip;]<\/p>\n","protected":false},"author":83,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-458","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.2 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Testing the cardiotoxicity of anti-cancer treatments on cardiac spheroids in vitro. - Service \/ FMPB - Cardiologie<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Testing the cardiotoxicity of anti-cancer treatments on cardiac spheroids in vitro. - Service \/ FMPB - Cardiologie\" \/>\n<meta property=\"og:description\" content=\"Our latest project is testing the cardiotoxicity of some anti-cancer treatments on in vitro cardiac spheroids developed in the laboratory (Ana\u00eblle Fratard&rsquo;s thesis). Developments in cancer treatments have improved the quality of patient care and their survival. However, some anti-cancer therapies are associated with toxicity, particularly cardiotoxicity. This leads to the development of cardiovascular disease [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/\" \/>\n<meta property=\"og:site_name\" content=\"Service \/ FMPB - Cardiologie\" \/>\n<meta property=\"article:modified_time\" content=\"2025-01-24T11:18:26+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-300x156.png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Dur\u00e9e de lecture estim\u00e9e\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/\",\"url\":\"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/\",\"name\":\"Testing the cardiotoxicity of anti-cancer treatments on cardiac spheroids in vitro. - Service \/ FMPB - Cardiologie\",\"isPartOf\":{\"@id\":\"https:\/\/web.umons.ac.be\/card\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-300x156.png\",\"datePublished\":\"2025-01-24T07:59:58+00:00\",\"dateModified\":\"2025-01-24T11:18:26+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/#breadcrumb\"},\"inLanguage\":\"fr-FR\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"fr-FR\",\"@id\":\"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/#primaryimage\",\"url\":\"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-300x156.png\",\"contentUrl\":\"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-300x156.png\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/web.umons.ac.be\/card\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Testing the cardiotoxicity of anti-cancer treatments on cardiac spheroids in vitro.\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/web.umons.ac.be\/card\/#website\",\"url\":\"https:\/\/web.umons.ac.be\/card\/\",\"name\":\"Service \/ FMPB - Cardiologie\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/web.umons.ac.be\/card\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"fr-FR\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Testing the cardiotoxicity of anti-cancer treatments on cardiac spheroids in vitro. - Service \/ FMPB - Cardiologie","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/","og_locale":"fr_FR","og_type":"article","og_title":"Testing the cardiotoxicity of anti-cancer treatments on cardiac spheroids in vitro. - Service \/ FMPB - Cardiologie","og_description":"Our latest project is testing the cardiotoxicity of some anti-cancer treatments on in vitro cardiac spheroids developed in the laboratory (Ana\u00eblle Fratard&rsquo;s thesis). Developments in cancer treatments have improved the quality of patient care and their survival. However, some anti-cancer therapies are associated with toxicity, particularly cardiotoxicity. This leads to the development of cardiovascular disease [&hellip;]","og_url":"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/","og_site_name":"Service \/ FMPB - Cardiologie","article_modified_time":"2025-01-24T11:18:26+00:00","og_image":[{"url":"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-300x156.png","type":"","width":"","height":""}],"twitter_card":"summary_large_image","twitter_misc":{"Dur\u00e9e de lecture estim\u00e9e":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/","url":"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/","name":"Testing the cardiotoxicity of anti-cancer treatments on cardiac spheroids in vitro. - Service \/ FMPB - Cardiologie","isPartOf":{"@id":"https:\/\/web.umons.ac.be\/card\/#website"},"primaryImageOfPage":{"@id":"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/#primaryimage"},"image":{"@id":"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/#primaryimage"},"thumbnailUrl":"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-300x156.png","datePublished":"2025-01-24T07:59:58+00:00","dateModified":"2025-01-24T11:18:26+00:00","breadcrumb":{"@id":"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/#breadcrumb"},"inLanguage":"fr-FR","potentialAction":[{"@type":"ReadAction","target":["https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/"]}]},{"@type":"ImageObject","inLanguage":"fr-FR","@id":"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/#primaryimage","url":"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-300x156.png","contentUrl":"https:\/\/web.umons.ac.be\/app\/uploads\/sites\/123\/2025\/01\/mise-en-paraffine-300x156.png"},{"@type":"BreadcrumbList","@id":"https:\/\/web.umons.ac.be\/card\/en\/testing-the-cardiotoxicity-of-anti-cancer-treatments-on-cardiac-spheroids-in-vitro\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/web.umons.ac.be\/card\/"},{"@type":"ListItem","position":2,"name":"Testing the cardiotoxicity of anti-cancer treatments on cardiac spheroids in vitro."}]},{"@type":"WebSite","@id":"https:\/\/web.umons.ac.be\/card\/#website","url":"https:\/\/web.umons.ac.be\/card\/","name":"Service \/ FMPB - Cardiologie","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/web.umons.ac.be\/card\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"fr-FR"}]}},"lang":"en","translations":{"en":458,"fr":454},"pll_sync_post":[],"_links":{"self":[{"href":"https:\/\/web.umons.ac.be\/card\/wp-json\/wp\/v2\/pages\/458","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/web.umons.ac.be\/card\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/web.umons.ac.be\/card\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/web.umons.ac.be\/card\/wp-json\/wp\/v2\/users\/83"}],"replies":[{"embeddable":true,"href":"https:\/\/web.umons.ac.be\/card\/wp-json\/wp\/v2\/comments?post=458"}],"version-history":[{"count":3,"href":"https:\/\/web.umons.ac.be\/card\/wp-json\/wp\/v2\/pages\/458\/revisions"}],"predecessor-version":[{"id":484,"href":"https:\/\/web.umons.ac.be\/card\/wp-json\/wp\/v2\/pages\/458\/revisions\/484"}],"wp:attachment":[{"href":"https:\/\/web.umons.ac.be\/card\/wp-json\/wp\/v2\/media?parent=458"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}