{"id":288,"date":"2022-08-20T11:26:27","date_gmt":"2022-08-20T09:26:27","guid":{"rendered":"https:\/\/web.umons.ac.be\/smos\/?p=288"},"modified":"2023-09-01T11:41:05","modified_gmt":"2023-09-01T09:41:05","slug":"deep-eutectic-solvents-for-the-extraction-and-stabilization-of-ecuadorian-quinoa-chenopodium-quinoa-willd-saponins","status":"publish","type":"post","link":"https:\/\/web.umons.ac.be\/smos\/2022\/08\/20\/deep-eutectic-solvents-for-the-extraction-and-stabilization-of-ecuadorian-quinoa-chenopodium-quinoa-willd-saponins\/","title":{"rendered":"Deep eutectic solvents for the extraction and stabilization of Ecuadorian quinoa (Chenopodium quinoa Willd.) saponins"},"content":{"rendered":"

In this work, a simple, fast (10\u00a0min) and environmentally friendly extraction method using deep eutectic solvents<\/a> (DES), based on choline<\/a> chloride and glycerol, was established to extract saponins from five quinoa samples: a) husks of bitter seeds, b) bitter seeds, c) water-washed bitter seeds, d) sweet seeds of the INIAP-Tunkahuan variety, and e) sweet seeds of the INIAP-Pata de Venado variety. The selected green solvent was a eutectic<\/a> mixture of choline chloride – glycerol – water at a 1:2:1\u00a0M ratio. The extractions were performed in a ball mixer<\/a> mill at room temperature (RT). A classical methanol – water (70:30, v\/v) extraction was performed for comparison. In all quinoa samples, the characterization and relative quantification of saponins were achieved based on mass spectrometry<\/a> analyses. Regardless of the type of solvent used (conventional or green solvents), hederagenin and phytolaccagenic acid were the major detected sapogenins in all tested quinoa samples. The husks of bitter seeds were the matrix with the highest saponin content. Although methanol – water (70:30, v\/v) was shown to extract three times more saponins compared to DES, the green solvent offered a higher stabilization of quinoa saponin liquid extracts – up to 2 months – compared to conventional solvents (ethanol – methanol – water; 67:23:10, v\/v\/v). The present research shows that DES represent an efficient green media for the stabilization of bioactive saponins from quinoa and have a potential as possible alter<\/p>\n

\"Relative
Relative amounts of saponins identified in the samples: water-washed bitter seeds, sweet seeds PV, and sweet seeds IT, extracted either with 70% methanol or DES2 (choline chloride – glycerol – water at a molar ratio 1:2:1). The solvent\/sample ratio is 20:1 (w\/w) to all samples. The structures of the different saponins are described in Table 1 n\u00a0=\u00a03 technical replicates.<\/figcaption><\/figure>\n

natives to organic solvents<\/a>. Our work opens new perspectives for the valorization of saponin-rich quinoa by-products (the husks of bitter seeds) as components for pharmaceutical, nutraceutical and agro-food applications.<\/p>\n

doi:\u00a010.1016\/j.jclepro.2022.132609<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

In this work, a simple, fast (10\u00a0min) and environmentally friendly extraction method using deep eutectic solvents (DES), based on choline chloride and glycerol, was established to extract saponins from five quinoa samples…<\/p>\n","protected":false},"author":157,"featured_media":289,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-288","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-non-classe"],"yoast_head":"\nDeep eutectic solvents for the extraction and stabilization of Ecuadorian quinoa (Chenopodium quinoa Willd.) saponins - Service \/ FS - Synth\u00e8se et Spectrom\u00e9trie de Masse Organiques<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/web.umons.ac.be\/smos\/2022\/08\/20\/deep-eutectic-solvents-for-the-extraction-and-stabilization-of-ecuadorian-quinoa-chenopodium-quinoa-willd-saponins\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Deep eutectic solvents for the extraction and stabilization of Ecuadorian quinoa (Chenopodium quinoa Willd.) saponins - 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